Kwasek Aquaculture Lab

Research

We continuously explore new research ventures. Over the years our lab has received many resources from state and federal agencies as well as from the industry (fish, feed, and raw material producers). Hence, we aim to tackle aquaculture challenges on a local, national, and global level. Our lab has worked with multiple species (trout, salmon, yellow perch, walleye, largemouth bass, tilapia, zebrafish, and shrimp) using both lab-based as well as hatchery-like research culture systems. Our efforts have been particularly placed on improving hatchery technology. Specifically, we have developed rearing systems destined for species characterized by challenging larval stage that ensure high survival, high swim-bladder inflation, low skeletal deformities, and reduced cannibalism. Although we continuously upgrade and improve our systems today we are able to achieve 50-70% survival of larval percids (up to 7 times higher compared to survival achieved in commercial hatcheries) with 100% swim-bladder inflation and 0% skeletal deformities.

Highlighted

Can human nutrition be improved through better fish feeding practices? a review paper
Can human nutrition be improved through better fish feeding practices? a review paper
Karolina Kwasek, Andrew L. Thorne-Lyman, Michael Phillips
Critical Reviews in Food Science and Nutrition  ·  25 Jan 2020  ·  doi:10.1080/10408398.2019.1708698

All

2025

Parental social environment has transgenerational effects on zebrafish (Danio rerio) growth
Parental social environment has transgenerational effects on zebrafish (Danio rerio) growth
Aubrey Dissinger, Karolina Kwasek
Scientific Reports  ·  07 Aug 2025  ·  doi:10.1038/s41598-025-13802-w
New approach to the development of tailor-made feed for fish larvae using Zebrafish Danio rerio as a model
New approach to the development of tailor-made feed for fish larvae using Zebrafish Danio rerio as a model
Giovanni S. Molinari, Michal Wojno, Macdonald Wick, Karolina Kwasek
PLOS One  ·  24 Jun 2025  ·  doi:10.1371/journal.pone.0326665

2024

Effects of dietary indispensable amino acid deficiencies on feed intake in stomachless fish
Effects of dietary indispensable amino acid deficiencies on feed intake in stomachless fish
Giovanni S. Molinari, Michal Wojno, Karolina Kwasek
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology  ·  01 Dec 2024  ·  doi:10.1016/j.cbpa.2024.111742
Effects of Oregano (<i>Origanum vulgare</i>) Essential Oil Supplementation on Growth Performance of Zebrafish (<i>Danio rerio</i>) Fed a High-Inclusion Soybean Meal Diet
Effects of Oregano (Origanum vulgare) Essential Oil Supplementation on Growth Performance of Zebrafish (Danio rerio) Fed a High-Inclusion Soybean Meal Diet
Aubrey Dissinger, Jacob Bowman, Giovanni S. Molinari, Karolina Kwasek
Zebrafish  ·  01 Oct 2024  ·  doi:10.1089/zeb.2024.0139
The Effect of the Species Source of Muscle and/or Digestive Enzymes on the Utilization of Fish Protein Hydrolysates as a Dietary Protein Source in First Feed for Larval Walleye (Sander vitreus)
The Effect of the Species Source of Muscle and/or Digestive Enzymes on the Utilization of Fish Protein Hydrolysates as a Dietary Protein Source in First Feed for Larval Walleye (Sander vitreus)
Giovanni S. Molinari, Michal Wojno, Genciana Terova, Macdonald Wick, Hayden Riley, Jeffery T. Caminiti, Karolina Kwasek
Animals  ·  28 Aug 2024  ·  doi:10.3390/ani14172493
Chronic social isolation affects feeding behavior of juvenile zebrafish (Danio rerio)
Chronic social isolation affects feeding behavior of juvenile zebrafish (Danio rerio)
Aubrey Dissinger, Simona Rimoldi, Genciana Terova, Karolina Kwasek
PLOS ONE  ·  26 Jul 2024  ·  doi:10.1371/journal.pone.0307967

2023

Partial and total replacement of soybean meal with a corn-fermented protein product in Rainbow Trout feeds
Partial and total replacement of soybean meal with a corn-fermented protein product in Rainbow Trout feeds
John Grayson, Erica Curles, Michal Wojno, Karolina Kwasek
North American Journal of Aquaculture  ·  20 Dec 2023  ·  doi:10.1002/naaq.10317
A Novel Approach in the Development of Larval Largemouth Bass Micropterus salmoides Diets Using Largemouth Bass Muscle Hydrolysates as the Protein Source
A Novel Approach in the Development of Larval Largemouth Bass Micropterus salmoides Diets Using Largemouth Bass Muscle Hydrolysates as the Protein Source
Giovanni S. Molinari, Michal Wojno, Genciana Terova, Macdonald Wick, Hayden Riley, Jeffrey T. Caminiti, Karolina Kwasek
Animals  ·  21 Jan 2023  ·  doi:10.3390/ani13030373

2022

Does Exposure of Broodstock to Dietary Soybean Meal Affect Its Utilization in the Offspring of Zebrafish (Danio rerio)?
Does Exposure of Broodstock to Dietary Soybean Meal Affect Its Utilization in the Offspring of Zebrafish (Danio rerio)?
Karolina Kwasek, Samuel Patula, Michal Wojno, Frank Oliaro, Chrissy Cabay, Lee J. Pinnell
Animals  ·  07 Jun 2022  ·  doi:10.3390/ani12121475
Feeding Rate and Protein Quality Differentially Affect Growth and Feeding Efficiency Response Variables of Zebrafish (<i>Danio rerio</i>)
Feeding Rate and Protein Quality Differentially Affect Growth and Feeding Efficiency Response Variables of Zebrafish (Danio rerio)
Shaley Valentine, Karolina Kwasek
Zebrafish  ·  01 Jun 2022  ·  doi:10.1089/zeb.2022.0002

2021

Fish muscle hydrolysate obtained using largemouth bass Micropterus salmoides digestive enzymes improves largemouth bass performance in its larval stages
Fish muscle hydrolysate obtained using largemouth bass Micropterus salmoides digestive enzymes improves largemouth bass performance in its larval stages
Karolina Kwasek, Christian Gonzalez, Macdonald Wick, Giovanni S. Molinari, Michal Wojno
PLOS ONE  ·  28 Dec 2021  ·  doi:10.1371/journal.pone.0261847
Proteomic profile and morphological characteristics of skeletal muscle from the fast- and slow-growing yellow perch (Perca flavescens)
Proteomic profile and morphological characteristics of skeletal muscle from the fast- and slow-growing yellow perch (Perca flavescens)
Karolina Kwasek, Young Min Choi, Hanping Wang, Kichoon Lee, John Mark Reddish, Macdonald Wick
Scientific Reports  ·  11 Aug 2021  ·  doi:10.1038/s41598-021-95817-7
The Effect of First Feeding Exposure of Larval Largemouth Bass to a Formulated Soybean Meal-Based or Soy Saponin-Supplemented Diet on Fish Growth Performance and Gut Microbiome
The Effect of First Feeding Exposure of Larval Largemouth Bass to a Formulated Soybean Meal-Based or Soy Saponin-Supplemented Diet on Fish Growth Performance and Gut Microbiome
Karolina Kwasek, Michal Wojno, Samuel Patula, Frank Oliaro, Chrissy Cabay, Lee J. Pinnell
North American Journal of Aquaculture  ·  10 Aug 2021  ·  doi:10.1002/naaq.10200
The use of dipeptide supplementation as a means of mitigating the negative effects of dietary soybean meal on Zebrafish Danio rerio
The use of dipeptide supplementation as a means of mitigating the negative effects of dietary soybean meal on Zebrafish Danio rerio
Giovanni S. Molinari, Michal Wojno, Vance J. McCracken, Karolina Kwasek
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology  ·  01 Jul 2021  ·  doi:10.1016/j.cbpa.2021.110958
The use of live food as a vehicle of soybean meal for nutritional programming of largemouth bass Micropterus salmoides
The use of live food as a vehicle of soybean meal for nutritional programming of largemouth bass Micropterus salmoides
Giovanni S. Molinari, Michal Wojno, Karolina Kwasek
Scientific Reports  ·  25 May 2021  ·  doi:10.1038/s41598-021-89803-2
Nutritional Programming with Dietary Soybean Meal and Its Effect on Gut Microbiota in Zebrafish (<i>Danio rerio</i>)
Nutritional Programming with Dietary Soybean Meal and Its Effect on Gut Microbiota in Zebrafish (Danio rerio)
Samuel Patula, Michal Wojno, Lee J. Pinnell, Frank Oliaro, Chrissy Cabay, Giovanni S. Molinari, Karolina Kwasek
Zebrafish  ·  01 Apr 2021  ·  doi:10.1089/zeb.2020.1952

2020

Can intestinal absorption of dietary protein be improved through early exposure to plant-based diet?
Can intestinal absorption of dietary protein be improved through early exposure to plant-based diet?
Giovanni S. Molinari, Vance J. McCracken, Michal Wojno, Simona Rimoldi, Genciana Terova, Karolina Kwasek
PLOS ONE  ·  04 Jun 2020  ·  doi:10.1371/journal.pone.0228758
Nutritional programming improves dietary plant protein utilization in zebrafish Danio rerio
Nutritional programming improves dietary plant protein utilization in zebrafish Danio rerio
Karolina Kwasek, Michal Wojno, Federica Iannini, Vance J. McCracken, Giovanni S. Molinari, Genciana Terova
PLOS ONE  ·  06 Mar 2020  ·  doi:10.1371/journal.pone.0225917
Can human nutrition be improved through better fish feeding practices? a review paper
Can human nutrition be improved through better fish feeding practices? a review paper
Karolina Kwasek, Andrew L. Thorne-Lyman, Michael Phillips
Critical Reviews in Food Science and Nutrition  ·  25 Jan 2020  ·  doi:10.1080/10408398.2019.1708698